Plant Tissue Culture

Employee Pattern

Our tissue culture laboratories will involve highly skilled and highly technical production methods and also with highly trained technical staff and scientists for monitoring their production. Depending upon the size of the production unit, the laboratory must require the scientists. visiting consultants for a particular technical problem will be employed.

Source of Ex-plant

Ex-plant is the portion of a disease-free and healthy plant procured from a healthy environment for the initiation process in the laboratory. Ex-plant is also called a mother plant because it is used for producing child-plants in the laboratory. The selection of a healthy and disease-free ex-plant was very important in commercial tissue culture production. Ex-plants were collected from a healthy plantation, from disease-free areas. The laboratory would collect local ex-plants for producing local varieties.

Total plantlets produced

OUR financial viability of the project depends mainly on the total production process. Total production was dependant on the installed capacity of the laboratory i.e. the production capacity of the laboratory in terms of plant production per year. Below is the chart where the actual production capacity of the laboratory and the actual production undertaken by the laboratory is compared. (Eg: Banana)

Technical back-up for the Lab

Setting up the laboratory is a very important task and should necessarily be handled by the technical expert in this field. Setting up of the laboratory involves deciding the location of the laboratory, purchasing fixed assets like land and building, Plant and Machinery and installing plant and machinery according to the laboratory production needs. Setting up a tissue culture laboratory also includes, designing the laboratory and the greenhouse, after deciding its installed capacity with the specified target of production. It also involves the procurement of miscellaneous items like chemical storage, greenhouse material, alternative power supply, etc. Designing and setting up the laboratory needs expertise and experience in this field. A novice trying to set up the laboratory uses the trial and error method, which requires a lot of time and energy and the entire project will not only get delayed but would prove to be costlier.

Bio-Tech Lab Equipments

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Reusable Plastic Labware

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A background to Tissue culture

Plant analysis often requires growing new seedlings in a controlled atmosphere. These may be plants that we have genetically modified in some way or maybe plants, of which we need many copies all specifically alike. These things can be accomplished into tissue culture of small tissue pieces from the plant of interest. These small parts may come from a single mother plant, or they may be the result of the genetic transmutation of individual plant cells, which are then rerouted to grow and to develop into a whole plant eventually. Tissue culture techniques are often used for industrial production of plants as well as for plant experimentation.

Tissue culture involves the use of small pieces of plant tissue (explants), which are cultured in a nutrient medium under sterile conditions. Using the appropriate growing conditions for each explant type, plants can be induced to produce new shoots rapidly and, with the addition of suitable hormones, new roots. These plantlets can also be divided, usually at the shooting stage, to produce large numbers of new seedlings. The new plants can then be placed in soil and grown healthily.

Many types of plants are suitable for use in the classroom. Cauliflower, rose cuttings, African violet leaves, and carnation stems will all easily produce clones (exact genetic copies) through tissue culture. Cauliflower florets, in particular, give excellent results since they can be grown into a complete plant in the primary tissue culture media, without the need for additional growth or root hormones. Green shoots are generally observable within three weeks, and roots develop within six weeks.

The most important part of this activity, however, is to maintain as sterile an environment as possible. Even one fungal spore or bacterial cell that comes into contact with the growth medium will rapidly reproduce and soon wholly overwhelm the small plant piece that you are trying to clone.

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